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Journal: Cell Genomics

Article Title: Variant-to-function analysis of the childhood obesity chr12q13 locus implicates rs7132908 as a causal variant within the 3′ UTR of FAIM2

doi: 10.1016/j.xgen.2024.100556

Figure Lengend Snippet:

Article Snippet: The guide RNA was then cloned into the gRNA_Cloning vector, which was a gift from George Church (Addgene Cat# 41824; http://n2t.net/addgene:41824; RRID: Addgene_41824), at the AflII restriction site with the NEB Gibson Assembly kit to generate the gRNA_Cloning-rs7132908gRNA vector.

Techniques: Recombinant, Saline, Modification, Transfection, Electron Microscopy, Membrane, Knock-Out, Gel Extraction, Plasmid Preparation, Mutagenesis, Reporter Assay, Sensitive Assay, Expressing, Purification, Software, Lysis, Cell Culture